Fig. 4: In vitro cytotoxicity of CD30 CAR-T cells.

The LDH assay of cytotoxicity of CD30 CAR T cells to Karpas 299 (A CD30+, **p < 0.01 vs. NC CART), SU-DHL-1 (B CD30+, **p < 0.01 vs. NC CART) and Jurkat (C CD30−); D the RTCA assay of the impedance of Karpas 299 cells, the reduction in impedance of Karpas 299 cells means more cells were killed by CD30 CAR T cells at E:T of 20:1, E the cytokine release of CD30 CAR T cells measured by Luminex 3D, F flow cytometry was used to evaluate the apoptosis rate of Karpas 299 cells, **p < 0.01 vs. NC CART.