Fig. 4: Insulin-like growth factor 1 receptor (IGF-1R) is a direct target of miR-625-5p in melanoma cells. | Cancer Gene Therapy

Fig. 4: Insulin-like growth factor 1 receptor (IGF-1R) is a direct target of miR-625-5p in melanoma cells.

From: Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression

Fig. 4

A The potential binding sites between the 3′-untranslated regions (UTRs) and the mutant sequences of miR-625-5p and IGF-1R are shown. B Luciferase activity was determined in A-375 and HT-144 cells cotransfected with miR-625-5p mimic or negative control (NC) mimic and IGF-1R-WT or IGF-1R-MUT. **P < 0.01 vs. group “NC mimic”. C IGF-1R mRNA level was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR) in 41 pairs of human melanoma tissues and corresponding adjacent normal tissues. **P < 0.01 vs. group “Normal”. D Correlation between miR-625-5p and IGF-1R expression in the 41 melanoma tissues was determined via Pearson correlation analysis. E, F mRNA and protein expression levels of IGF-1R in miR-625-5p-overexpressed A-375 and HT-144 cells was measured by qRT-PCR and western blotting, respectively. **P < 0.01 vs. group “NC mimic”.

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