Fig. 5: Long intergenic non-protein coding RNA 1291 (LINC01291) decoys miR-625-5p and promotes insulin-like growth factor 1 receptor (IGF-1R) expression in melanoma cells. | Cancer Gene Therapy

Fig. 5: Long intergenic non-protein coding RNA 1291 (LINC01291) decoys miR-625-5p and promotes insulin-like growth factor 1 receptor (IGF-1R) expression in melanoma cells.

From: Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression

Fig. 5

A, B Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were used to measure IGF-1R expression in A-375 and HT-144 cells after si-LINC01291 or non-targeted siRNA introduction. **P < 0.01 vs. group “si-NC”. C Pearson correlation analysis was performed to assess the association between LINC01291 and IGF-1R levels in the 41 melanoma tissues. D Radioimmunoprecipitation (RIP) assay was conducted to validate the binding interaction between LINC01291, miR-625-5p, and IGF-1R in melanoma cells. **P < 0.01 vs. group “IgG”. E qRT-PCR was used to explore the efficiency of anti-miR-625-5p transfection in A-375 and HT-144 cells. **P < 0.01 vs. group “anti-NC”. F, G Anti-miR-625-5p or anti-NC was transfected into LINC01291-deficient A-375 and HT-144 cells. The changes in the mRNA and protein expression levels of IGF-1R were determined via qRT-PCR and western blotting, respectively. **P < 0.01 vs. groups “si-NC” and “si-LINC01291 + anti-NC”.

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