Fig. 7: Overexpressing insulin-like growth factor 1 receptor (IGF-1R) counteracts the effects of long intergenic non-protein coding RNA 1291 (LINC01291) silencing in melanoma cells. | Cancer Gene Therapy

Fig. 7: Overexpressing insulin-like growth factor 1 receptor (IGF-1R) counteracts the effects of long intergenic non-protein coding RNA 1291 (LINC01291) silencing in melanoma cells.

From: Long noncoding RNA LINC01291 promotes the aggressive properties of melanoma by functioning as a competing endogenous RNA for microRNA-625-5p and subsequently increasing IGF-1R expression

Fig. 7

A Western blotting was performed to detect the expression of IGF-1R protein in A-375 and HT-144 cells after pc-IGF-1R or pcDNA3.1 transfection. **P < 0.01 vs. “pcDNA3.1” group. B, C Cell Counting Kit-8 (CCK-8) and colony formation assays were conducted to examine the proliferation and colony formation ability of A-375 and HT-144 cells after cotransfection of pc-IGF-1R or pcDNA3.1 and si-LINC01291. **P < 0.01 vs. groups “si-NC” and “si-LINC01291 + pcDNA3.1”. D, E Flow cytometry was performed to measure the apoptotic rate and cell cycle status of the aforementioned cells. **P < 0.01 vs. groups “si-NC” and “si-LINC01291 + pcDNA3.1”. F, G Cell migration and invasive abilities of cells treated with si-LINC01291 along with pc-IGF-1R or pcDNA3.1 were observed using cell migration and invasion assays. **P < 0.01 vs. groups “si-NC” and “si-LINC01291 + pcDNA3.1”.

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