Fig. 2: MiR-328-3p overexpression dampens breast cancer metastasis. | Cancer Gene Therapy

Fig. 2: MiR-328-3p overexpression dampens breast cancer metastasis.

From: Fatty acid β-oxidation promotes breast cancer stemness and metastasis via the miRNA-328-3p-CPT1A pathway

Fig. 2

A The relative expression of miR-328-3p in MDA-MB-468 and MDA-MB-231 cells with mimic-miR-328-3p. Mean ± SD, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 3. B The relative expression of miR-328-3p in MDA-MB-468, MDA-MB-231 and MCF-7 with inhibitor miR-328-3p. Mean ± SD, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 3. C, D The invasion ability of MDA-MB-468 cells transfected with mimic-miR-328-3p was detected by Transwell assay. Panel C is a representative images, and panel D is a relative statistic graph. Scale bar: 50 μm. Mean ± SD, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 3. E, F The invasion ability of MCF-7 with miR-328-3p inhibition was detected by the Transwell assay. Representative images (E) and statistical graphs (F) are shown. Bar: 50 μm. Mean ± SD, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 3. G The invasion ability of MDA-MB-231 with miR-328-3p inhibition was detected by the Transwell assay. Bar: 50 μm. H Wound closure was evaluated using the equation described in the materials and methods. Values are the mean ± SD of three independent experiments. *p < 0.05 by one-way ANOVA with Dunnett t-test.

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