Fig. 5: MiR-328-3p controls fatty acid β-oxidation via CPT1A. | Cancer Gene Therapy

Fig. 5: MiR-328-3p controls fatty acid β-oxidation via CPT1A.

From: Fatty acid β-oxidation promotes breast cancer stemness and metastasis via the miRNA-328-3p-CPT1A pathway

Fig. 5

A, B Lipid droplet levels in MDA-MB-468 cells with mimic-miR-328-3p was detected by BODIPY 493/503 staining. Representative images are shown in A, the fluorescence foci were quantified and shown in B. Scale bar: 20 μm. Mean ± SD, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 12 in three independent experiment. C, D The lipid droplet levels in MDA-MB-468 cells transduced with inhibitor miR-328-3p with or without Etomoxir were evaluated by BODIPY 493/503 staining. Representative images (C) and fluorescence foci quantification (D) are shown. Scale bar: 20 μm. Mean ± SD, *p < 0.05, ***p < 0.001 by one-way ANOVA with Dunnett t-test, n = 12 in three independent experiment. E–H Change in OCR (ΔOCR) following palmitate-BSA addition calculated as (OCR at the time of palmitate-BSA injection—final basal OCR) in MDA-MB-231 and MDA-MB-468 with miR-328-3p knockdown (E, F) or overexpression (G, H). Mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA with Dunnett t-test (n = 3).

Back to article page