Fig. 7: MiR-328-3p depletion enhances cancer cells stemness. | Cancer Gene Therapy

Fig. 7: MiR-328-3p depletion enhances cancer cells stemness.

From: Fatty acid β-oxidation promotes breast cancer stemness and metastasis via the miRNA-328-3p-CPT1A pathway

Fig. 7

A The relative ALDH1 expression in MDA-MB-468 cells with overexpressed miR-328-3p by RT-qPCR. Mean ± SD, ***p < 0.001 by one-way ANOVA with Dunnett t-test (n = 3). B The proportions of ALDH1+ cancer cells were monitored by flow cytometry, cells were treated with or without mimic-miR-328-3p in MDA-MB-468 CSCs spheroids. Mean ± SD, **p < 0.01 by One-way ANOVA with Dunnett t-test (n = 3). C The co-expression of cytokeratin (CK) and ALDH1 in clinical BC tissue with low or high miR-328-3p expression. Scale bar: 50 μm. D, E The spheroid forming ability of MDA-MB-468 (D) or MCF-7 (E) in the indicated treatment. Scale bar: 30 μm. F Scheme of the miR-328-3p-mediated cell stemness network in BC: The low expression of miR-328-3p in small partition BC patients who suffered tumor metastasis, which promotes CPT1A expression that enhances fatty acid β-oxidation and stemness of cells, then promotes cancer cell metastasis.

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