Fig. 1: The combination therapy induces effective tumour growth inhibition and long-term survival benefit in 4T1 breast cancer.

A Mice were treated as shown in the scheme. B Tumour volume of different treatment groups. C Kaplan–Meier plot for overall survival. D Comparisons of tumour growth curves over time. At the end of the experiment, intact tumours and lungs were removed. E Tumour weight (n = 10 for each group). F Tumour lesions in the lungs were counted (n = 5 in each group). G Lung slices were subjected to H&E staining to confirm metastasis. The statistical chart and representative images are shown (n = 6 for each group). The data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control; #p < 0.05, ##p < 0.01, ####p < 0.0001 versus aPC; &p < 0.05 versus corresponding oncolytic viruses. $p < 0.05 rAd.GM versus rAd.Null. One-way ANOVA followed by Bonferroni post hoc tests was used.