Fig. 3: Combination therapy regulated lymphocyte infiltration as well as TAM polarization and immune-related genes in the TME. | Cancer Gene Therapy

Fig. 3: Combination therapy regulated lymphocyte infiltration as well as TAM polarization and immune-related genes in the TME.

From: Oncolytic adenoviruses synergistically enhance anti-PD-L1 and anti-CTLA-4 immunotherapy by modulating the tumour microenvironment in a 4T1 orthotopic mouse model

Fig. 3

A Panel 1 of the multiplex immunohistochemistry detection of T cell infiltration in 4T1 tumour tissue (scale bars = 50 μm). B The percentages of CD4+ and CD8+ T cells, Tregs and memory T cells. C Panel 2 of the multiplex immunohistochemistry detection of immune checkpoint expression and macrophage polarization. D The percentages of CD274+ (PD-L1) and CD152L+ (CTLA-4) cells, TAMs and M1 and M2 macrophages. E On day 24, tumours were removed, and total RNA was isolated. After cDNA was synthesized, the expression of Th1 cytokines (IL-2 and INF-γ), Th2 cytokines (TGF-β), chemokines (CXCL10) and cytotoxicity-related genes (granzyme B and perforin) was analysed by real-time RT-PCR and normalized to β-actin (n = 4/group). The data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 versus control; #p < 0.05, ##p < 0.01, ###p < 0.001, ####p < 0.0001 versus aPC; &p < 0.05, &&p < 0.01, &&&p < 0.001, &&&&&p < 0.0001 versus corresponding oncolytic viruses. One-way ANOVA followed by Bonferroni post hoc tests was used.

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