Table 1 The gene ID and primers’ sequences used in RT-PCR experiments.

From: Oncolytic adenoviruses synergistically enhance anti-PD-L1 and anti-CTLA-4 immunotherapy by modulating the tumour microenvironment in a 4T1 orthotopic mouse model

Gene

Gene ID

Direction

Sequence (5’−3’)

Actin—Mouse

11461

Forward

AGGCCAACCGTGAAAAGATG

Reverse

TGGCGTGAGGGAGAGCATAG

IL-2—Mouse

16183

Forward

TGAGTGCCAATTCGATGA

Reverse

AGGGCTTGTTGAGATGATGC

IL-10—Mouse

16153

Forward

AGTGGTATAGACAGGTCTGTTGG

Reverse

GCAGCTCTAGGAGCATGTGG

IFN-γ—Mouse

15978

Forward

ACTGGCAAAAGGATGGTG

Reverse

GTTGCTGATGGCCTGATT

CCL5—Mouse

20304

Forward

ATATGGCTCGGACACCAC

Reverse

GTGACAAACACGACTGCAA

Granzyme B—Mouse

14939

Forward

TGAAGTCAAGCCCCACTC

Reverse

TCAGCACAAAGTCCTCTCG

Perforin—Mouse

18646

Forward

CTGGGATGCCGACTACG

Reverse

CACCCTGCCGTGGTTTA

CXCL10—Mouse

15945

Forward

TTTCTGCCTCATCCTGCT

Reverse

CCCTATGGCCCTCATTCT

TGF-β—Mouse

21803

Forward

CTCCCGTGGCTTCTAGTGC

Reverse

GCCTTAGTTTGGACAGGATCTG

Actin—Homo

6774

Forward

GGACTGAGCATCGAGCA

Reverse

GCCAGACCCAGAAGGAG

IL-2—Homo

3558

Forward

AGAACTCAAACCTCTGGAGGAAG

Reverse

GCTGTCTCATCAGCATATTCACAC

IL-10—Homo

3586

Forward

ACCAAGACCCAGACATCAA

Reverse

CATTCTTCACCTGCTCCAC

Granzyme B—Homo

3002

Forward

CCAGGGCATTGTCTCCTA

Reverse

GGGGCTTAGTTTGCTTCC