Fig. 3: Comparison of CXCR4 expression levels between liver metastatic murine models and subcutaneous mouse models. | Cancer Gene Therapy

Fig. 3: Comparison of CXCR4 expression levels between liver metastatic murine models and subcutaneous mouse models.

From: Non-invasive detection and complementary diagnosis of liver metastases via chemokine receptor 4 imaging

Fig. 3

A In vitro UM cell lines with high (+++, 91.6% positive, top left), intermediate (++, 30.7% positive, middle left), and low (+,13.6% positive, bottom left) expression levels of CXCR4 by flow cytometry were used for generation of metastatic UM mice models. The CXCR4 expression levels obtained by IHC in primary eye UM was intermediate in M20-09-196 mice (top middle), and low in OMM2.3 (middle) and OCM1 mice (bottom middle). The CXCR4 expression levels by IHC in hepatic metastases was high in all three mice models (top right, middle right and bottom right) regardless of the CXCR4 expression levels in vitro and in primary ocular UM. B Comparison of CXCR4 IRS between ocular UM and UM hepatic metastases from M20-09-196, OMM2.3, and OCM1 mice models. The CXCR4 expression (represented by IRS) was significantly elevated in hepatic UM in comparison with the primary ocular UM in M20-09-196 (p < 0.05), OMM2.3 (p < 0.01), and OCM1 mice models (p < 0.01). C H&E staining (left), gp100 IHC staining(middle), and CXCR4 staining (right) of subcutaneous UM tumors generated from M20-09-196 and Mel290 cells. D Quantitative comparison of CXCR4 IRS between subcutaneous UM tumor from M20-09-196 and Mel290.

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