Fig. 4: TRPV1 overexpression promoted rapid growth and invasiveness in lung cancer cells. | Cancer Gene Therapy

Fig. 4: TRPV1 overexpression promoted rapid growth and invasiveness in lung cancer cells.

From: RETRACTED ARTICLE: Overexpressed transient receptor potential vanilloid 1 (TRPV1) in lung adenocarcinoma harbours a new opportunity for therapeutic targeting

Fig. 4

(A) The proliferation of A549 or TRPV1-A549 cells influenced by TRPV1 siRNA (5 nM, for 48 h) or CPZ (25 μM, for 1 h) was detected by MTT assay. (B) Cytoplasmic cyclin A and PCNA expression influenced by TRPV1 siRNA or CPZ were measured by western blot. (C) The Mean Optical Density (MOD) of bands for Cyclin A and PCNA were visualized and analyzed using Image J software. (D), (E) The migration of A549 or TRPV1-A549 cells influenced by TRPV1 siRNA or CPZ was detected by trans-well migration assay. (F) Cytoplasmic MMP1, MMP9, RhoA, Rac1, and Cdc42 expression influenced by TRPV1 siRNA or CPZ were measured by western blot. (G) The MOD value of MMP1, MMP9, RhoA, Rac1, and Cdc42 were visualized and analyzed using Image J software. Data were expressed as Mean ± SEM (n = 3). *Represents significant difference as compared with control group, *p < 0.05, **p < 0.01.

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