Fig. 2: Progestin-PR signaling-mediated secretion of paracrine factors by ESCs inhibited EC cell proliferation. | Cancer Gene Therapy

Fig. 2: Progestin-PR signaling-mediated secretion of paracrine factors by ESCs inhibited EC cell proliferation.

From: NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB

Fig. 2

A CM derived from MPA-pretreated ESCs and MPA cotreatment showed the greatest inhibitory effect on EC cell growth compared with 20 μM MPA group and CM (ESCs) + MPA group. CM was extracted from ESCs treated with or without 10 μM MPA for 48 h. Ishikawa and ECC-1 cells were cultured with CM and/or 10 μM MPA for 48 h before CCK-8 assays. B ESCs were transfected with siRNAs targeting PGR. Real-time PCR and western blotting were used to evaluate PGR mRNA and PR protein (PRA and PRB) expression. C, D The inhibitory effect of CM (ESCs-siPGR + MPA) on EC cell proliferation was compromised compared with CM (ESCs + MPA) group. Ishikawa and ECC-1 cells were cultured with the indicated CM for 48 h before CCK-8 and EdU assays. Scale bar, 300 μm. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant.

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