Fig. 3: Progestin-PR signaling in ESCs increased PR expression in EC cells via hydroxymethylation of the PRB promoter. | Cancer Gene Therapy

Fig. 3: Progestin-PR signaling in ESCs increased PR expression in EC cells via hydroxymethylation of the PRB promoter.

From: NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB

Fig. 3

A, B CM (ESCs-siPGR + MPA) attenuated the upregulation of CM (ESCs + MPA) on PGR mRNA and PR protein expression in EC cells. CM was extracted from ESCs and ESCs-siPGR with or without 10 μM MPA treatment for 48 h. Then Ishikawa and ECC-1 cells were treated with the indicated CM for 24 or 48 h, respectively. PGR mRNA and PR protein expression were analyzed by real-time PCR and western blotting, respectively. GAPDH was used as the normal control. C CM (ESCs-siPGR + MPA) attenuated the upregulation of CM (ESCs + MPA) on genomic DNA hydroxymethylation. Then Ishikawa and ECC-1 cells were cultured with the indicated CM for 24 h. 5-hmC levels of total genomic DNAs were assessed by dot blot analysis. D CM (ESCs-siPGR + MPA) attenuated the upregulation of CM (ESCs + MPA) on hydroxymethylation levels of the PGR promoter in EC cells. After 24 h of treatment with the indicated CM, Ishikawa and ECC-1 cells were harvested to assess 5-hmC levels in the PRB promoter fragment. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant.

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