Fig. 6: NrCAM inhibited EC xenograft growth. | Cancer Gene Therapy

Fig. 6: NrCAM inhibited EC xenograft growth.

From: NrCAM secreted by endometrial stromal cells enhances the progestin sensitivity of endometrial cancer cells through epigenetic modulation of PRB

Fig. 6

A A single-cell suspension of 5 × 105 cells was injected into the uterine horns of nude mice. After 14 days, the mice were treated with 5 mg/kg NrCAM in PBS (i.p.) every 2 d and/or 100 mg/kg MPA in corn oil by gavage at a dose of 0.1 mL/10 g daily for another 16 days, and then were sacrificed. B, C NrCAM+MPA had the highest inhibitory effect on EC uterine horn xenograft growth compared with NrCAM or MPA alone. Images of uterine horn xenografts (B). Weight of uterine horn xenografts in different groups (C). Ruler unit, 1 mm. D IHC staining for Ki67, PR, and 5-hmC in EC xenograft tumor tissues. The IHC staining score was calculated by semi-quantitative optical analysis. Ki67 score (n = 5 each group; NrCAM + MPA versus MPA, P < 0.001; NrCAM + MPA versus NrCAM, P < 0.001); 5-hmC score (n = 5 each group; NrCAM+MPA versus MPA, P < 0.001; NrCAM +MPA versus NrCAM, P < 0.001); PR score (n = 5 each group; NrCAM+MPA versus MPA, P < 0.001; NrCAM +MPA versus NrCAM, P < 0.01). Original magnification, ×200. CDX, cell-derived xenograft. *P < 0.05; **P < 0.01; ***P < 0.001; n.s. not significant.

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