Fig. 1: Identification of key genes using a shRNA library screen approach. | Cancer Gene Therapy

Fig. 1: Identification of key genes using a shRNA library screen approach.

From: Functional characterization of the PI3K/AKT/MTOR signaling pathway for targeted therapy in B-precursor acute lymphoblastic leukemia

Fig. 1

A Schematic representation of the shRNA library screen. RCH-ACV leukemia cells (E2A-PBX1+/pre-BCR+) were transduced with shRNA sublibraries. After puromycin selection, the frequency of individual shRNAs was quantified at day 0 and at day 12 by deep sequencing. The experiment was performed in duplicate. B Dot plot shows Mann–Whitney U test p values for enrichment (increase proliferation) and depletion (decrease proliferation) of targeted genes by shRNA knockdown and analyzed by deep-sequencing. Each dot represents a gene. C Small drug screen. RCH-ACV cells were treated with several small molecule inhibitors and viable cells were enumerated after four days. Graph shows half inhibitory growth concentration (IC50). At least three independent experiments were performed per treatment.

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