Fig. 4: miR-590-3p directly targets TRAIP and represses TRAIP expression.

A The binding sites of miR590-3p and TRAIP 3′-UTR. B Luciferase analysis showed the inhibitory effect of miR-590-3p on expression of the luciferase reporter gene by binding TRAIP 3′-UTR. C Western blot showed the TRAIP protein was highly up-regulated in miR-590-3p inhibitor and down-regulated by miR-590-3p mimic. All data are shown as the means ± SD of three experiments. (**P < 0.01).