Fig. 5: TRAIP reverses the effects of miR-590-3p on cells proliferation and induces G1/S arrest via RB-E2F signaling. | Cancer Gene Therapy

Fig. 5: TRAIP reverses the effects of miR-590-3p on cells proliferation and induces G1/S arrest via RB-E2F signaling.

From: Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT

Fig. 5

A CCK-8 assay was examined 1, 2, 3, 4, and 5 days in MDA-MB-231 (Control group, miR-590 inhibitor group, miR-590 inhibitor+ShTRAIP-1 group) and BT-549 (Control group, miR-590 mimic group, miR-590 mimic + OETRAIP group). B Colony formation were photographed and colony numbers were illustrated in histogram. C Flow cytometry revealed the distribution of cell phase in MDA-MB-231 and BT-549 cell lines. D The expression of RB, phospho-RB, E2F1, CyclinE1, Cyclin D1 and P21 was examined by Western blot. All data are shown as the means ± SD of three experiments. (**P < 0.01, *P < 0.05, Control vs. miR-590-3p inhibitor, or Control vs. miR-590-3p mimic; ##P < 0.01, #P < 0.05, miR-590 inhibitor vs. miR-590 inhibitor+ShTRAIP-1, or miR-590 mimic vs. miR-590 mimic+OETRAIP).

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