Fig. 4: YAP m5C modification increases exosome concentration. | Cancer Gene Therapy

Fig. 4: YAP m5C modification increases exosome concentration.

From: YAP 5-methylcytosine modification increases its mRNA stability and promotes the transcription of exosome secretion-related genes in lung adenocarcinoma

Fig. 4

A–B Exosome concentration was measured in A549 and H1299 cells with YAP overexpressed (A) or knocked out (B), with or without NSUN2 and ALYREF overexpressed. C The exosome from H1299 cells with YAP, NSUN2 with or without ALYREF overexpression was marked by PKH67 (green) and incubated with A549 cells. Afterwards, IF was performed for the detection of exosome. Scale bar, 50 µm. D Representative TEM images of plasma exosome in A549 and H1299 cells. Scale bar, 300 nm. E Relationship between exosome concentration and size in A549 and H1299 cells. The mean and mode size of exosome were shown. F Exosome concentration in A549 and H1299 cells. G Relationship between exosome concentration and size in A549 cells with YAP, NSUN2 with or without ALYREF overexpression. The mean and mode size of exosome were shown. The data are shown as the means ± SD from 3 to 5 independent experiments. *p < 0.05, **p < 0.01 indicate statistical significance. NS, non-significant. The data from (A–C) were analyzed by a one-way ANOVA test. The data from (E, G) were analyzed by a two-way ANOVA test. The data from (F) were analyzed by a student’s t test.

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