Fig. 1: lncNAP1L6 promoted cell migration, invasion and EMT in PCa. | Cancer Gene Therapy

Fig. 1: lncNAP1L6 promoted cell migration, invasion and EMT in PCa.

From: LncNAP1L6 activates MMP pathway by stabilizing the m6A-modified NAP1L2 to promote malignant progression in prostate cancer

Fig. 1

A RT-qPCR examine lncNAP1L6 expression in PCa cell lines (22RV1), PCa bone metastasis cell lines (PC3, C4-2B and VCaP) and PCa brain metastasis cell line (DU145). B LncNAP1L6 was knocked down in PC3 and VCaP cells. C LncNAP1L6 was overexpressed in PC3 and VCaP cells. D, E Wound healing assays evaluated the migration of PCa cells. F Transwell assays assessed cell migration and invasion when lncNAP1L6 was downregulated. G Cell migration was observed through transwell assays when lncNAP1L6 was upregulated. H Western blot analyzed the protein levels of ZO-1, E-cadherin, N-cadherin and Vimentin after lncNAP1L6 was silenced. I Western blot analyzed the protein levels of E-cadherin, Vimentin and Fibronectin after lncNAP1L6 was overexpressed. *P < 0.05, **P < 0.01.

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