Fig. 7: KCNF1 positively regulates ITGB4 expression and downstream signaling.
From: KCNF1 promotes lung cancer by modulating ITGB4 expression
A Lysates of A549 and H23 cells transduced with lentiviral vectors expressing a control shRNA or an shRNA against KCNF1 were analyzed by anti-KCNF1, anti-ITGB4, anti-p-FAK, anti-FAK, anti-p-AKT, anti-AKT, or anti-Actin. B Lysates of A549 and Beas2B cells with forced expression of FLAG and HA-tagged KCNF1 (FH-KCNF1) were analyzed by immunoblotting using anti-HA, anti-ITGB4, anti-p-FAK, anti-FAK, anti-p-Akt, anti-Akt, or anti-Actin. C A549 cells transduced with shControl or shKCNF1were transfected with a control vector or a vector expressing Myc-His-ITGB4. Levels of ITGB4, Myc-His-ITGB4, p-FAK, FAK, p-Akt, Akt, KCNF1, Actin, and GAPDH were analyzed by immunoblotting.
