Fig. 1: Maltonis-mediated cell-free chromatin complexes formation and H3K9me3 reorganization. | Cancer Gene Therapy

Fig. 1: Maltonis-mediated cell-free chromatin complexes formation and H3K9me3 reorganization.

From: Small molecule-induced epigenomic reprogramming of APL blasts leading to antiviral-like response and c-MYC downregulation

Fig. 1

A Maltonis structure. B DNA-histones cross-linking induced by maltonis. Native chromatin or free DNA plus histone proteins were incubated for 4 h at 37 °C with maltonis or with formaldehyde (FA) at the concentrations reported in figure. DNA-protein complexes were then isolated by biochemical fractionation and the DNA in chromatin-containing fractions was fluorimetrically quantified. C + (FA 4 mM): positive control. The experiment was conducted in triplicate and the statistical significance of each experimental condition, using chromatin or DNA and histones, was evaluated using student’s t-test (two-sided), p value < 0.01. C Evaluation of global H3K9me3 changes induced by maltonis in NB4 cells. NB4 cells were treated with the reported concentrations of maltonis and total cell lysates were analyzed by western blot.

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