Fig. 2: Genome-wide analysis of the gene expression modulations induced by maltonis in NB4 cells.

NB4 cells were subjected to maltonis treatment at the concentration of 10 µM for 24 h or left untreated and analyzed by RNA-seq. Biological triplicates were produced for both maltonis-treated and untreated cells. A Hierarchical clustering heatmap of the most variable 10% genes between maltonis treated and not treated (n.t.) cells. As reported in the color key, positive Z-scores (green) are associated with higher expression values while negative Z-scores (red) indicate a lower expression value. n.t.: not treated cells. B Principal component analysis (PCA) of the most variable 10% expressed genes between not treated (n.t.) and maltonis-treated cells. C Volcano plot of differential expressed genes (DEGs) in consequence of maltonis treatments in NB4 cells. Genes from differential gene expression analysis were identified as differentially expressed (DEGs) when the following criteria were met: log₂(FC) > 1.32, FDR < 0.01, and RPKM_Treat > 10 for upregulated DEGs, while for downregulated DEGs, log₂(FC) < −1.32, FDR < 0.01, and RPKM_Crtl > 10. Upregulated genes are shown in green while downregulated genes are shown in red. The total number of transcripts analyzed and the one found to be regulated are reported at the top of the graph. D Gene set enrichment analysis (GSEA) showing the most upregulated (in green) and downregulated (in red) hallmark gene sets.