Fig. 1: KDM5B transcripts and protein isoforms. | Cancer Gene Therapy

Fig. 1: KDM5B transcripts and protein isoforms.

From: A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression

Fig. 1

A KDM5B transcripts as reported in Ref Seq NCBI database. RBP2-H1, PLU-1, KDM5B-240, and KDM5B-215 are the validated (NM) variants, X1 and X2 are the predicted (XM) ones. Transcripts differ at the 5′ UTRs and for the presence of alternative exons. RBP2-H1 represents the longest transcript and includes the exon-6 (in red). The arrows indicate the ATG sites, the first one in exon-1 and the second one in exon-4. B Protein domains of human KDM5B are reported above validated and predicted KDM5B protein isoforms; PLU-1 is the canonical isoform. Ab1 and Ab2 are KDM5B C-terminus and exon-6 specific antibodies, respectively. C WB analysis of KDM5B isoform expression in MCF7 lysates: on the left two bands corresponding to 175.7 and 161.5 kDa proteins are detected by Ab1 in the first blot, while on the right one single band for 161.5 kDa protein is detected by Ab2, after the stripping of the first blotting. D Preliminary investigation of KDM5B isoforms’ expression in different human cell lines and human testis, using the two antibodies Ab1 (panel up) and Ab2 (panel down), before and after the stripping, respectively. Panels A and B created with BioRender.com.

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