Fig. 2: 5′RLM-RACE analysis of KDM5B transcripts. | Cancer Gene Therapy

Fig. 2: 5′RLM-RACE analysis of KDM5B transcripts.

From: A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression

Fig. 2

A The scheme reports the position of reverse primers used for the two RT-PCRs of 5′RACE experiment. Binding nucleotides are indicated underneath RBP2H1 sequence. See Material and Methods paragraph for experimental details. B PCR products from PCR1 and PCR2. Product lengths are as expected. C The position of first and second ATG codons are shown with green arrows. We found a new 5′-end 221 nucleotides downstream the canonical one. D Partial alignment of PCR1 and PCR2 sequences refer to KMD5B-PLU1 transcript. (For the complete alignment see Supplementary File S1). Panel A created with BioRender.com.

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