Fig. 5: MS-based analysis of H3K4 methylation levels in MCF7 and MDA-MB-231 cells overexpressing the KDM5B-NTT isoform. | Cancer Gene Therapy

Fig. 5: MS-based analysis of H3K4 methylation levels in MCF7 and MDA-MB-231 cells overexpressing the KDM5B-NTT isoform.

From: A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression

Fig. 5

A The KDM5B-NTT expression in the overexpression condition in MCF7 cells at 24 h from transfection increases compared to cells transfected with the Empty vector (E), where the KDM5B-NTT expression is similar to that observed in the non-transfected cells (NT). B The KDM5B-NTT expression increases also in MDA-MB-231 cells at 24 h from transfection in the overexpression condition compared to negative controls. C Heatmap display of the log2 L/H ratios (where L: samples and H: internal standard) obtained for H3K4 methylations in MCF7 and MDA-MB-231 cells not transfected (NT), transfected with empty vector (E), or overexpressing KDM5B-NTT. The data were normalized over the average value across all the samples. D H3K4me2 and H3K4me3 levels (expressed as L/H ratios) in MCF7 and MDA-MB-231 cells overexpressing KDM5B-NTT, compared to control E. Statistical analysis was performed by repeated measures ANOVA, followed by Tukey’s multiple comparison test. **p < 0.005, ***p < 0.001.

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