Fig. 6: Effects of KDM5B-NTT overexpression on transcriptome. | Cancer Gene Therapy

Fig. 6: Effects of KDM5B-NTT overexpression on transcriptome.

From: A truncated and catalytically inactive isoform of KDM5B histone demethylase accumulates in breast cancer cells and regulates H3K4 tri-methylation and gene expression

Fig. 6

A, B Volcano plots showing the differentially expressed genes (DEGs) in MCF7 and MDA-MB-231 cells with overexpression of KDM5B-NTT, respectively. Red dots indicate DEGs associated with p < 0.1 and log2(FC) < −1 or log2(FC) > 1; blue dots indicate genes with p < 0.1 and −1 < log2(FC) < 1; green dots indicate genes with log2(FC) < −1 or log2(FC) > 1, and p > 0.1. C Heatmap showing in color scale the GSEA Normalized Enriched Score (NES) of gene expression profile resulting from overexpression of KDM5B-NTT in MCF7 and MDA-MB-231 cells in comparison with control cell samples. (White * = FDR < 0.01). D Cluster of KDM5B-NTT-deregulated genes, which are also significantly induced by one or both epigenetic drug (PDCA and MTA) and/or previously shown to be bound by KDM5B in ChIP experiments [19, 22, 25].

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