Fig. 3: YAP1 perturbation blunts STK11-loss-dependent cytokine induction upon glutamine depletion in human KRAS-driven lung adenocarcinoma cell lines. | Cancer Gene Therapy

Fig. 3: YAP1 perturbation blunts STK11-loss-dependent cytokine induction upon glutamine depletion in human KRAS-driven lung adenocarcinoma cell lines.

From: STK11 loss leads to YAP1-mediated transcriptional activation in human KRAS-driven lung adenocarcinoma cell lines

Fig. 3

A Western blot analysis confirming knockout of YAP1 (ΔY) in NCI-H2009 and NCI-H441 parent (P) and STK11-KO (ΔS) cell lines. The STK11/YAP1 double knockout lines are abbreviated as ΔSY. B IL-6, CXCL8, and CXCL2 qRT-PCR analysis demonstrates that upon glutamine depletion, the STK11-loss-dependent induction is blunted by the absence of YAP1. Expression normalized to PSMB4, and data presented as mean ± SD (N = 3). C IL6 and CXCL8 ELISAs performed on conditioned media from H2009 cell lines. Data presented as mean ± SD (N = 3). D qRT-PCR analysis of IL-6, CXCL8, and CXCL2 on cells treated with 1.5 mM verteporfin (VP) vs vehicle. Expression normalized to PSMB4, and data presented as mean ± SD (N = 3). *p < 0.0332, **p < 0.0021, ***p < 0.0002, ****p < 0.0001 was calculated by two-way ANOVA and Tukey test in (B, C) or three-way ANOVA and Tukey test in (D).

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