Fig. 2: Long-term hypoxia adaptation induces an miRNA signature linked to EMT. | Cancer Gene Therapy

Fig. 2: Long-term hypoxia adaptation induces an miRNA signature linked to EMT.

From: Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform

Fig. 2

A Hierarchically clustered heatmap of all differentially expressed (Benjamini-Hochberg adjusted p-value < 0.05 in the 5% vs 0.1% O2 comparison, and > 100 normalized DESeq2 reads in any condition) miRs, normalized to contribution to total expression in the dataset. Red denotes the classical hypoxia-induced miR, miR-210-3p. Green denotes EMT-promoting miRs miR-221/222. Blue and purple denote other miRs of interest. Heatmap clustered using WardD.2 hierarchical linkage metric, with the number of clusters chosen subjectively. B Expression values for miR-210-3p. C Expression values for miR-125b-1-3p. D Expression values for miRs of interest in cluster 4. E Expression values for miRs of interest in cluster 5. F, G Expression values for genes Cpeb1 and Sema6d. B, G Expression levels are DESeq2 normalized reads. * denotes relative significance as calculated by DESeq2 Benjamini-Hochberg adjusted p-value (padj). *padj < 0.05, **padj < 0.01, ***padj < 0.001.

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