Fig. 5: Novel truncated Wt1 transcripts encode efficiently translated proteins that accumulate in the nucleus. | Cancer Gene Therapy

Fig. 5: Novel truncated Wt1 transcripts encode efficiently translated proteins that accumulate in the nucleus.

From: Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform

Fig. 5

A Splicing events observed in LTHY data. Percentages are the average between replicates, coverage depths are overlaid. B Potential open reading frames (ORFs) derived from the tWt1 intron 5 sequence in E7 isoforms. Purple: Intron 5 derived sequence. Orange: Exon 7 derived sequence. Bold: Canonical WT1 ORF (third ORF). Bright-green/dark-green: In/out of frame start codons. Red: Stop codons. C Possible tWt1 isoforms. Purple: Intronic sequence. Orange: Exonic sequence. Blue: KTS motif. D GFP levels of DOX-induced expression of tWT1-GFP isoforms in B16 cells. E Microscopy images of tWT1-GFP fusion constructs. Nuclear staining was performed using Hoechst 33342 (Thermo FIsher: H1399) as per the manufacturers protocol. F Western Blot of HEK cells expressing DOX inducible GFP or E7K-tWT1-GFP. Top: anti-Calnexin. Bottom: anti-GFP. G Mass Spectrometry (MS) coverage of E7K-tWt1 purified from HEK cells. Refer to the legend for full annotation.

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