Fig. 6: Novel E7-tWT1 isoform retains DNA binding ability and is associated with genes involved in EMT. | Cancer Gene Therapy

Fig. 6: Novel E7-tWT1 isoform retains DNA binding ability and is associated with genes involved in EMT.

From: Long-term severe hypoxia adaptation induces non-canonical EMT and a novel Wilms Tumor 1 (WT1) isoform

Fig. 6

A Left: top: schematic of cWt1 CDS. GFP was linked C-terminally as per the E7-GFP construct. Bottom: microscopy image of cWt1 under Dox induction. Right: GFP induction levels of cWt1 relative to E7-GFP. Induction was performed after 36 h of incubation at 0.5% O2, as per the LTHY protocol. All Dox inductions were performed at 2 µg/mL. B Known and de novo TF motif analysis of E7-K tWT1 ChIPseq data. The known motif p-value = 1e−78, is found in 36% of called peaks. The de novo motif p-value = 1e−105, motif is found in 30% of ChIPseq peaks. C, D Functional annotation bubbleplots of ChIPseq called peaks. E E7-tWT1 ChIPseq coverage and LTHY RNAseq expression profiles for genes of interest. cWt1 and input chromatin were used as negative controls. Black arrow denotes CDS start. * denotes relative significance as calculated by DESeq2 Benjamini-Hochberg adjusted p-value (padj). *padj < 0.05, **padj < 0.01, *** padj < 0.001.

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