Fig. 1: NLCs and M2-THP1 cells release extracellular vesicles into their growth medium, which are efficiently incorporated into CLL-B-cells. | Cancer Gene Therapy

Fig. 1: NLCs and M2-THP1 cells release extracellular vesicles into their growth medium, which are efficiently incorporated into CLL-B-cells.

From: Extracellular vesicles from type-2 macrophages increase the survival of chronic lymphocytic leukemia cells ex vivo

Fig. 1

A EVs were isolated by ultracentrifugation from Nurse-Like Cells (NLCs) culture medium, their size was evaluated (mode (nm) ±SEM) by NTA using the NanoSight NS3000 (Malvern Panalytical). In (B), a similar analysis was conducted for EVs isolated from M2-THP1 culture medium. C Western blot analysis was performed to assess exosomal (CD9, CD81, and CD63) and macrophage (CD68) markers on NLCs and M2-THP1 cells lysate and EVs isolated from the culture medium of NLCs and M2-THP1 cells. D Time-course analysis by flow cytometry of PKH67 labeled EVs incorporation into CLL-B-cells (0.001 ng of EVs per cell). E Fluorescence microscopy of CLL-B-cells incubated for 2 h with PKH67-labeled EVs isolated from the culture medium of NLCs and M2-THP1 cells, or without EVs (x63 magnification).

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