Fig. 2: Mutating of the YxxxYY-motif in TrkB restores actin filopodia dynamics. | Cancer Gene Therapy

Fig. 2: Mutating of the YxxxYY-motif in TrkB restores actin filopodia dynamics.

From: Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene

Fig. 2

A, B Overexpression of TrkB kinase induces round-shaped cell morphology and loss of actin filopodia dynamics. HEK293 cells co-expressing GFP-actin and TrkB-wt (in A) or TrkB-YFF mutants (in B). Time-lapse images (in seconds) are shown. Living cells were imaged using a confocal laser-scanning microscope. GFP-labeled-actin was excited with a 488 nm laser, and fluorescence was detected using a spectral detector (510–570 nm). A Arrows point to a roundish cell that forms typical blebs. B The arrow indicates typical dynamic filopodia labeled by GFP-actin. Videos can be found in the supplement. Scale bar: 25 µm.

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