Table 3 Primer sequences.

From: Atypical cellular responses mediated by intracellular constitutive active TrkB (NTRK2) kinase domains and a solely intracellular NTRK2-fusion oncogene

Primer name

Reference sequence

Amplicon (in reference)

Primer sequence

hTrkB splicea

NM_006180

(position 1836–2019)

5´– CTGTGGTGGTGATTGCGTCT –3´

3´– GGGCTGGCAGAGTCATCATC –5´

RNApol II (POLR2A)

NM_000937

(position 4466–4732)

5´– GCACCACGTCCAATGACAT –3´

3´– GTGCGGCTGCTTCCATAA –5´

  1. aThe primers span two splice sites in the human NTRK2 gene. This includes the splice site for TrkB-kinase versus truncated TrkB-T1.
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