Fig. 1: Interference with exogenous reporter gene expression in HEK-293T cells via the HOPE strategy.
From: NF-κB-activated oncogene inhibition strategy for cancer gene therapy
A Representative fluorescence images of HEK-293T cells at 24 h post transfection. pHOPE-NT/mCherry/ZsGreen, HOPE plasmids targeting no transcript and the mCherry and ZsGreen reporter gene transcripts. Phase, bright field; ZsGreen/mCherry, green fluorescent/red fluorescent field; scale bar, 50 μm. B Bar chart showing the numbers of cells exhibiting fluorescence. Cells exhibiting fluorescence were counted in the corresponding fluorescence images with ImageJ2 software (n = 3 images). C The mRNA expression of reporter genes was measured by qPCR. ZsGreen and mCherry gene expression was measured by qPCR (n = 3 wells). RQ = 2–ΔΔCt; RQ relative quantification.
