Fig. 5: Effect of HOPE treatment on cancer stem cells in the TME.
From: NF-κB-activated oncogene inhibition strategy for cancer gene therapy
A Schematic workflow for the data collection and scRNA-seq analysis to define the changes in the TME caused by HOPE treatment. B UMAP plot of cell types derived from the HOPE-treated and PBS non-treated samples. The cells are coloured according to cell type. C Bubble plot showing genes (columns) that were differentially expressed across various cell types (rows). The scale bar depicts low expression in white and high expression in red. The percentages of cells expressing specific genes are represented by the sizes of the circles. D UMAP plot of the HOPE-treated and PBS non-treated samples. The cell types are represented by different colours. E Sankey diagram showing the proportion of each cell type in the HOPE-treated and PBS non-treated samples. F Comparison of significant DEGs in each cell type between the HOPE-treated and PBS non-treated samples. The significantly upregulated genes in the HOPE-treated samples are shown with red dots. The blue dots represent genes significantly upregulated in the non-treated samples. G The pathways significantly enriched with the DEGs. Biological pathways in the MSigDB Hallmark database were used for enrichment analysis. Blue represents pathways enriched in PBS non-treated samples, and red represents pathways enriched in the HOPE-treated samples. H The numbers of intercellular communication interactions among cancer cells and other cell types in the HOPE-treated and PBS non-treated samples. The line thickness represents the number of interactions. HOPE-treated, rAAV-HOPE-treated; non-treated, PBS-treated.
