Fig. 3: Tob acts as a negative regulator of TNF-α-induced NF-κB activation. | Cancer Gene Therapy

Fig. 3: Tob acts as a negative regulator of TNF-α-induced NF-κB activation.

From: Tob negatively regulates NF-κB activation in breast cancer through its association with the TNF receptor complex

Fig. 3

MCF-7 cells were transiently transfected with a control, Tob siRNA-1 (a, b) or siRNA-2 (c, d). After 72 h, cells were treated with 10 ng/mL TNF-α. Bar graphs show the pIκB-α/ IκB-α in three independent experiments (Supplementary Fig. 2a–d). e Primary Tob+/+ or Tob-/- MEFs were treated with 20 ng/mL TNF-α. f MCF-7 were transiently transfected with a pME18s control or Tob expression vector. After 24 h, cells were treated with 10 ng/mL TNF-α. Values in western blotting figures indicate the band intensity of phosphorylated proteins divided by that of total proteins. These results indicate the mean ± S.D. (n = 3). Statistical significance was assessed using Student’s t-test. *p < 0.05, **p < 0.01.

Back to article page