Fig. 4: Tob downregulates transcriptions regulated by NF-κB. | Cancer Gene Therapy

Fig. 4: Tob downregulates transcriptions regulated by NF-κB.

From: Tob negatively regulates NF-κB activation in breast cancer through its association with the TNF receptor complex

Fig. 4

MCF-7 cells were transfected with a control, Tob siRNA-1 (a) or siRNA-2 (b). After 72 h, cells were treated with 10 ng/mL TNF-α. Expression levels of Il8 and RelB were measured with real-time RT-PCR. c Primary Tob+/+ or Tob-/- MEFs were treated with 20 ng/mL TNF-α. Expression levels of RelB and Nfkbia were measured with real-time RT-PCR. d MCF-7 parental, Tob KO, or Tob KO transfected with Tob expression vector was treated with 10 ng/mL TNF-α⊡ The expression level of Il8 and Nfkbia was measured with real-time RT-PCR. mRNA expression levels were normalized against Gapdh in each sample. Fold induction was calculated by dividing expression values by that of control siRNA, Tob+/+ or MCF-7 parental without TNF-α stimulation. These results indicate the mean ± S.D. (n = 3 (a, b, c), n = 4 (d), technical replicates). Statistical significance was assessed using Student’s t-test. *p < 0.05, **p < 0.01.

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