Fig. 1: Expression of lncRNAs in the HGG TIME in RCAS/t-va immunosuppressive and pro-inflammatory models. | Cancer Gene Therapy

Fig. 1: Expression of lncRNAs in the HGG TIME in RCAS/t-va immunosuppressive and pro-inflammatory models.

From: Identification of an immunomodulatory lncRNA signature associated with immune cell reprogramming in high-grade glioma

Fig. 1

A Schematic representation of the TIME in HGG RCAS murine models: RCAS-PDGFb develops a “cold” TIME, while RCAS-BRAF V600E develops a “hot” TIME, also characterized by infiltration of immunosuppressive myeloid cells and exhausted T cells (biorender.com). B Left: Dendrogram of the unsupervised hierarchical clustering analysis of total RNA sequencing in intratumoral immune cells from RCAS-PDGFb HGG mice (n = 4) compared with age-matched no-tumor controls (n = 3). Right: Volcano plot of differentially expressed lncRNAs in tumor-infiltrating immune cells (3665 genes in total). C Left: Dendrogram of the unsupervised hierarchical clustering analysis of total RNA sequencing in intratumoral immune cells from RCAS-BRAF V600E HGG mice (n = 3) compared with age-matched no-tumor controls (n = 3). Right: Volcano plot of differentially expressed lncRNAs in tumor-infiltrating immune cells (2819 genes in total). B, C Genes significantly upregulated are highlighted in , and those significantly downregulated are in . D Venn diagram showing the overlap of differentially expressed lncRNAs in the TIME of the HGG RCAS-PDGFb and HGG RCAS-BRAF V600E models. Numbers indicate lncRNAs unique to each model or shared between them. E Heatmaps of the immunoregulatory lncRNAs differentially expressed in tumor-infiltrating immune cells compared with no-tumor controls. F Hypothesis model illustrating the proposed role of lncRNAs in regulating the TIME (biorender.com). Statistical significance was calculated using an unpaired two-tailed Student’s t-test (P < 0.05).

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