Fig. 4: Targeting mTORC2 inhibits glycolysis and lactate secretion.

Seahorse analysis of (A) extracellular acidification rate (ECAR), (B) glycolysis, glycolytic capacity, and glycolytic reserve and (C) in vitro lactate concentrations in HCC2814 WT vs MLST8-KO. Seahorse analysis of (D) extracellular acidification rate (ECAR), (E) glycolysis, glycolytic capacity, and glycolytic reserve and (F) in vitro lactate concentrations in H596 WT vs MLST8-KO. Seahorse analysis of (G) extracellular acidification rate (ECAR), (H) glycolysis, glycolytic capacity, and glycolytic reserve and (I) in vivo tumor interstitial lactate concentrations in KLN205-Ptennull WT vs Mlst8-KO. All data are presented as mean ± SEM from two or three independent experiments. p values were determined by two-tailed unpaired Student t test. *p < 0.05, **p < 0.01.