Fig. 1: The mTOR inhibitor rapamycin improves the differentiation of FAP1-hESCs into colon organoids.
From: Rapamycin rescues APC-mutated colon organoid differentiation
A A schematic diagram of the colon differentiation protocol from hESCs, with the indicated timing of rapamycin treatment. B Representative images of 42-48 days 3D colon organoids derived from heterozygous mutated APC FAP1-hESCs cultured with and without rapamycin treatment. H&E staining presents the complexity of the organoids following rapamycin treatment. C, D The number of complex colon organoids developed by day 48 of differentiation of FAP1-hESCs cultured with and without rapamycin treatment. A total of 14 biological experiments were performed and 26–31 Matrigel drops were analyzed. Graphs present the average of complexed organoids per one Matrigel drops, values are presented as Avg ± Std. *P < 0.05, ****P < 0.001; t test. E Organoids area size of a representative FAP1-colon organoids section measured using the image processing program ImageJ, of 38 untreated and 35 rapamycin-treated organoids. Values are presented as Avg ± Std; ****P < 0.0001 (t test).
