Fig. 6: FAP1 differentiated cells inhibit apoptosis through the inactivation of cMyc.
From: Rapamycin rescues APC-mutated colon organoid differentiation
A FACS analysis plots of apoptotic cells, analyzed by Annexin-V (x-axis, FITC-A), and necrotic cells analyzed by PI (y-axis, PE-A), at day 20 of differentiation, using the MEBCYTO Apoptosis Kit (Annexin V-FITC Kit). A total of 10,000 cells of each line were analyzed and shown is a representative plot. B, C Quantification of FACS analysis for apoptosis (B) and live cells (C), of three independent experiments. ****P < 0.0005, t test. D Immunohistochemical analysis of c-Myc expression in day 45 FAP1 colon organoids with/without rapamycin (±R). Normal colon tissue served as a negative control. This experiment was repeated twice, with the analysis of 2–3 pathological sections.
