Fig. 3: Characterization of isogenic platinum resistant HGSOC cell lines.
A IC50 values for cisplatin and (B) carboplatin comparing the parental platinum sensitive cell line (left) to its isogenic resistant pair(s) (right). Line and error bars for the sensitive cell lines and resistant pairs show mean +/- standard error of the mean. Cmax range is shown with the yellow rectangle. C Heatmap of WNT/EMT, STAT, and Platinum Influx/Efflux gene expression from RNA-seq. Genes were selected based on combination of magnitude of change across all differential gene expression datasets and literature relevance to platinum. Expected directionality of change associated with resistance is shown on far left. Asterisks indicate adjusted p-values < 0.05, with larger asterisks indicating directionality consistent with resistance. D CLOVAR differentiated gene signature score for individual RNA-seq replicates. Asterisks indicate adjusted p-value < 0.05 with unpaired t-test. E Gene ontology analysis showing overrepresented pathways in the differentially expressed genes found when comparing the sensitive and resistant isogenic pairs individually. Gene ontology results are aggregated by semantic space to shared pathways, where the number of individual gene ontology terms is indicated in the label in parentheses. Each isogenic pair is color-coded independently, as indicated in the legend.
