Fig. 4: Markers of stemness and wound healing in isogenic platinum resistant HGSOC cell lines. | Cancer Gene Therapy

Fig. 4: Markers of stemness and wound healing in isogenic platinum resistant HGSOC cell lines.

From: Cell-intrinsic platinum response and associated genetic and gene expression signatures in ovarian cancer

Fig. 4

A Wound healing assay for the PEA1/PEA2 (N = 3 independent replicates), OVCAR3 (N = 4), OVCAR4 (N = 4), and PEO1/4 (N = 3) isogenic cell line pairs. Error bars represent standard deviation. * indicates p-value < 0.05 in paired t-tests and ns indicates non-significant p-value > 0.05. B Representative ALDH1A1 western blot images of all isogenic cell line pairs along with platinum resistant cell lines JHOS2 and COV362. GAPDH serves as a loading control. C CD133 and ALDH1A1 western blot images of all isogenic cell line pairs with or without cisplatin treatment at each respective cell line’s IC50 for 72 hours. GAPDH serves as a loading control. D Volcano plot showing differential expression between isogenic platinum-resistant cell lines, comparing resistant to their sensitive counterpart. Points are color-coded based on the specific pair, and statistically significant ( | log2 fold change |>1.5, adjusted p-value < 0.05) genes are labeled by gene name.

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