Fig. 3

Diminished PPARG expression alters the adipogenic potential of p53^−/− MSCs. a PPARG protein and mRNA expression quantified by qPCR (upper panel) and immunoblot analyses. The blot for this panel is a duplication from Figure 1i by mistake.  Dr. Shi requested that we add the correct blot panel.  Therefore, I have created a revised figure 3R2 with the corrected panel. Not sure I can attach it here but will e-mail if not (bottom panel), respectively, in p53^+/+ vs. p53^−/− MSCs. QPCR data are mean ± S.D. of experiments (n = 3) repeated twice. ***p < 0.005 by Student’s t-test. b, d PPARG mRNA and protein expression quantified by qPCR (upper panel) and immunoblot analyses (bottom panel), respectively, in MSCs transfected with a scrambled (Scr) and p53-specific siRNA (b) or Scr and Pparg-specific siRNA (d). QPCR data are mean ± S.D. of experiments (n = 3) repeated twice. ***p < 0.005 by Student’s t-test. c ChIP showing pull-down of Pparg promoter sequences using a RNA Pol II (positive control) or p53 antibody from cell extracts of p53^+/+ MSCs treated with Nutlin-3a (20 µM). *p < 0.05, **p < 0.01 compared to IgG control by ANOVA and Tukey post hoc test. e qPCR of Pparg mRNA levels in MSCs transfected with a p53-specific siRNA. Data are mean ± S.D. of experiments (n = 3) repeated three times. ***p < 0.005 by Student’s t-test. f Effect of siRNAs on CFU-F activity in MSCs. Data are mean ± S.D. of experiments (n = 6). ***p < 0.005 by Student’s t-test. g Effect of siRNAs on stimulus-induced adipogenic (left panel) and osteogenic (right panel) differentiation. Data are mean ± S.D. of experiments (n = 4). **p < 0.01 compared to CCM, ⁺p < 0.05 vs. Scr + AIM, and ⁺⁺p < 0.01 vs.Scr + OIM by one-way ANOVA and Tukey post hoc test