Fig. 6
From: Basal p53 expression is indispensable for mesenchymal stem cell integrity
FGF2 modulates the cellular redox balance via regulation of TWIST2. a, b Histograms (left panel) and percentage of MitoSOX Red high cells (right panel) from flow cytometric analysis of MitoSOXTM Red-stained p53+/+ MSCs transfected with the indicated siRNAs (a) or cultured in CCM supplemented with or without FGF2 (20 ng/ml) under 5 or 21% oxygen saturation (b). Data are mean ± S.D. of experiments run in duplicate. *p < 0.05 compared to Scr, ++p < 0.01 compared to Twist1 by Student’s t-test. *p < 0.05 compared to CCM, +p < 0.05 compared to FGF2 treated by ANOVA and Tukey post hoc test. c QPCR analysis of Twist2 mRNA levels in p53+/+ MSCs from (b). Data are mean ± S.D. of experiments (N = 3) repeated twice. **p < 0.01 compared to CCM, #p < 0.01 vs. FGF2 by ANOVA and Tukey post hoc test. d Immunoblots (left panel) and densitometry (right panels) showing TWIST2, BAX, and p53 protein expression in cells from (b). e qPCR analysis of Twist2 mRNA levels in p53−/− MSCs treated as in (b). Data are mean ± S.D. of experiments (N = 3) repeated twice and normalized to data shown in (c). *p < 0.05, **p < 0.01 compared to CCM, #p < 0.01 vs. FGF2 by ANOVA and Tukey post hoc test. f Percentage of MitoSOX Red high cells based on flow cytometric analysis of MitoSOXTM Red-stained p53−/− MSCs cultured in CCM supplemented with or without FGF2 (20 ng/ml) under 5 or 21% oxygen saturation. Data are mean ± S.D. of experiments run in duplicate. *p < 0.05 compared to CCM by ANOVA and Tukey post hoc test. g qPCR analysis of normalized Fgf2 mRNA levels in p53−/− vs. p53+/+ MSCs. Data are mean ± S.D. of experiments (n = 3) repeated twice. ***p < 0.005 by Student’s t-test
