Fig. 1

BOK contributes to DEN-induced liver damage. a Bok mRNA expression in the livers of WT and Bok^-/- animals after DEN exposure. Hprt served as reference gene. Values represent the mean ± SD (n = 3 mice per group). b Serum ALT levels were determined in WT and Bok^-/- mice after DEN exposure (100 mg/kg BW). Values represent mean ± SD. c Representative fluorescence micrographs of TUNEL staining and d quantification of TUNEL-positive cells in livers from WT and Bok^-/- animals treated with DEN (100 mg/kg BW). Values represent the mean ± SD (n = 3 mice per group). e Quantitative western blot analysis using near-infrared fluorescence of activated caspase-3 in liver lysates from WT and Bok^-/- animals 3 days after DEN (100 mg/kg) injection. Values represent the mean ± SD (n = 3 mice per group)