Fig. 2
From: BOK promotes chemical-induced hepatocarcinogenesis in mice
BOK protein is increased in response to DEN and promotes induction of CHOP, BIM and PUMA. a Quantitative western blot analysis showing increase in BOK protein levels in WT animals treated with DEN (100 mg/kg). Lower band of BOK is due to alternative START codon [20]. Values represent mean ± SD (n = 3–6 per group). Statistical analysis was performed by one-way ANOVA followed by a Tukey post-test analysis. b Induction of Bim and Puma mRNA by DEN (100 mg/kg) is decreased in livers of Bok-/- mice. Gapdh served as reference gene. Values are normalized to PBS controls and represent the mean ± SD (n = 3 mice per group). c Representative western blot data of liver lysates from DEN (100 mg/kg) treated WT and Bok-/- mice and quantification using near-infrared fluorescence. Values represent the mean ± SD (n = 6 mice per group)
