Fig. 2: Endogenous DG accumulation triggers ROS production and necrosis

a Growth curve of the TKO in comparison to wild type (Wt). Administration of 1 mM choline chloride increases the growth rate of the TKO. b Time course survival assay based on clonogenicity of yeast cells. c Flow cytometry-assisted analysis of DHE to ethidium conversion for ROS quantification measured 20 h after inoculation. d Microscopy pictures of the same samples used in c. Scale bar = 10 µm. e Flow cytometry-assisted analysis of AnnexinV (green)/ PI (red) co-staining for differentiation between apoptosis and necrosis measured 20 h after inoculation. p-values were calculated for the PI positive fractions. f Representative microscopy images of the same samples as used in e. Scale bar = 5 µm. For panels a and b statistical significance was assessed using two-way ANOVA with time and strain as independent factors. Panel c and d were analyzed using one-way ANOVA. ANOVA analysis in d refers to the PI-only-positive fraction shown in gray. Error bars indicate SEM and asterisks in the figures indicate significant differences, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001