Fig. 4: DG-mediated cell death depends on glucose and hexokinase 2 mediated glucose repression

The time course of clonogenic survival a and ROS production b of wild-type yeast grown on glucose or galactose as carbon sources was determined with and without DOG administration. c Clonogenic survival assay of hexokinase deletion mutants (hxk1∆ and hxk2∆) with and without DOG treatment. Clonogenic survival assay d and ROS assessment e comparing TKO hxk2∆ to TKO and wild-type (Wt) cells. f Clonogenic survival plating and g PI-measurement of hxk2 mutants. S14D represents the phospho-mimetic mutant, whereas mutant S14A is not subject to phosphorylation at position 14 anymore. Statistical significance in panels a and b was assessed using three-way ANOVA with carbon source, treatment and time as independent factors. c, f and g were analyzed using two-way ANOVA with strain and treatment as independent factors. Error bars indicate SEM and asterisks in the figures indicate significant differences, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001